| CAS Number | 74103-06-3 |
|---|---|
| Molecular Formula | C15H13NO3 |
| Molecular Weight | 255.273 |
| InChI Key | OZWKMVRBQXNZKK-UHFFFAOYSA-N |
| LogP | 2.35 |
| Synonyms |
|
Applications:
HPLC Chiral Separation of Ketorolac Enantiomers on Cyralis CM Column
August 20, 2026
HPLC Chiral Method for Analysis of Ketorolac Enantiomers on Cyralis CM Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ketorolac.
Ketorolac is a nonsteroidal anti-inflammatory drug (NSAID) with the chemical formula C15H13NO3. It is used primarily used to treat moderate to severe pain. Due to a high risk of negative effects, including mortal gastrointestinal bleeding, it is only taken for short intervals of time. Despite the adverse effects, it is a widely available and often prescribed in the United States.
This application describes a normal-phase chiral HPLC method for the separation of Ketorolac enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.
Ketorolac can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and TFA. Detection is performed using UV.
Condition
| Column | Cyralis CM, 4.6 x 100 mm, 5 µm, 100 A, normal phase |
| Mobile Phase | Hexane/Ethanol/TFA |
| Flow Rate | 1.0 mL/min |
| Detection | UV 320 nm |
| Resolution | 1.59 |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Ketorolac |
Application Column
Cyralis CM
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase
Separation of Ketorolac on Newcrom R1 HPLC column
May 16, 2018
Ketorolac can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options