| CAS Number | 466-99-9 |
|---|---|
| Molecular Formula | C17H19NO3 |
| Molecular Weight | 285.343 |
| InChI Key | WVLOADHCBXTIJK-YNHQPCIGSA-N |
| LogP | 1.24 |
| Synonyms |
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Applications:
HPLC Separation of Morphine and Hydromorphone on Primesep 100 Column
August 3, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis Morphine and Hydromorphone
Morphine and Hydromorphone are opioid medications. Morphine works by blocking pain signals from traveling along the nerves to the brain. Hydromorphone is a semisynthetic derivative of morphine. Morphine and Hydromorphone can be retained and separated on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Morphine and Hydromorphone |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsMorphine
Separation of Hydromorphone on Newcrom R1 HPLC column
May 16, 2018
Hydromorphone can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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