HPLC Method for Analysis of Diazinon on Newcrom BH Column

HPLC Method for Diazinon on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Diazinon

Diazinon is an insecticide with the chemical formula C12H21N2O3PS. Due to it’s high toxicity to vertebrates which leads to headaches, vomiting, apnea, and more, it has been banned in the United States since 2004. You can find detailed UV spectra of Diazinon and information about its various lambda maxima by visiting the following link.

Diazinon can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnNewcrom BH, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 30/70%
BufferH2SO4 – 0.1%
Flow Rate0.5 ml/m
DetectionUV 194 nm
Class of Compounds
 Insecticide
Analyzing CompoundsDiazinon

Application Column

Newcrom BH

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Diazinon
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.