HPLC Method for Oligonucleotides on Chromni by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Oligonucleotides
Oligonucleotide 5A is a small RNA molecule with a specific adenine sequence. They are primarily used to inhibit viral and cancer cell growth. In RNA synthesis, they are intermediates in enzymatic synthesis of capped RNAs.
Oligonucleotides can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium acetate as a buffer. Detection is performed using UV.
| Column | Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated |
| Mobile Phase | Gradient MeCN – 75 – 55%, 10 min |
| Buffer | Ammonium Acetate pH 5.0 – 10mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 |
| Class of Compounds | Oligonucleotide |
| Analyzing Compounds | Oligonucleotides |
Application Column
Chromni
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated
Application Analytes:
OligonucleotidesApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
