HPLC Method for Pyrazine, 2-Aminopyrazine, Pyrazinamide, 3-Aminopyrazole-4-carboxylic acid on Primesep A by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pyrazine, 2-Aminopyrazine, Pyrazinamide, 3-Aminopyrazole-4-carboxylic acid.

Pyrazinamide is a medication used in the treatment of infectious disease tuberculosis and is on the World Health Organization’s (WHO) list of essential medicines. It has the chemical formula C₅H₅N₃O.

Pyrazine is an organic compound with C₄H₄N₂ chemical formula. It is known for its sweet and nutty aroma, but is usually used as a therapeutic agent in several drugs. Derivatives of it are also said to have anti-inflammatory, anticancer, antibacterial, and antioxidant properties.

Aminopyrazine, also known as 2-aminopyrazine and pyrazinamide, is a Pyrazine derivative with C₄H₅N₃ chemical formula. It is typically used as a building block in chemical synthesis and is found in pharmaceuticals, especially  in antiviral drugs and other agrochemicals. Structure wise, it is a pyrazine ring with an attached amine group.

It can be retained and separated from other pyrazine compounds, which are structurally similar and can be difficult to separate in reverse-phase HPLC, by using Primesep A mixed-mode column. Primesep A’s stationary phase is embedded with strong acidic ion-pairing groups. The analytical method is isocratic and uses the mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) as buffer and UV detection at 275nm.