HPLC Separation of Pyrazinecarboxamide and Related Compounds


HPLC.cloud
View on hplc.cloud
Pyrazinamide is a medication used in the treatment of infectious disease tuberculosis and is on the World Health Organization’s (WHO) list of essential medicines. It can be retained and separated from other pyrazine compounds, which are structurally similar and can be difficult to separate in reverse-phase HPLC, by using Primesep A mixed-mode column. Primesep A’s stationary phase is embedded with strong acidic ion-pairing groups. The analytical method is isocratic and uses the mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) as buffer and UV detection at 275nm.

Condition
Column Primesep A, 4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV 275 nm

Description
Class of Compounds Hydrophilic,  Heterocyclic, Aromatic
Analyzing Compounds Pyrazine,  Aminopyrazine, Pyrazinamide, 3-Aminopyrazole-4-carboxylic acid

 

Application Column

Primesep A

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2-Aminopyrazine
3-Aminopyrazole-4-carboxylic acid
Pyrazinamide
Pyrazine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.