HPLC Method for Analysis of Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid on Newcrom BH by SIELC Technologies

Glyoxylic Acid is the smallest α-keto acid with the formula CHO-COOH. It is a yellowish solid that occurs naturally and is useful industrially. It participates in many biochemical reactions. Its reduced form, D-lactic acid, can be found in various microorganisms. You can find detailed UV spectra of Glyoxylic Acid and information about its various lambda maxima by visiting the following link.

Glycolic Acid is the smallest α-hydroxy acid (AHA) with the formula HOCH₂COOH. It’s derived from sugar cane and is a colorless, odorless, and hygroscopic crystalline solid. It is used in various skin-care products because of its capability to penetrate skin. You can find detailed UV spectra of Glycolic Acid and information about its various lambda maxima by visiting the following link

Acetic Acid is a simple carboxylic acid with the formula CH₃COOH. It’s an important chemical reagent and industrial chemical, primarily used in the production of cellulose acetate for photographic film and polyvinyl acetate for wood glue, as well as synthetic fibers and fabrics. It gives vinegar its sour taste and pungent smell. It is well known for being the active ingredient in vinegar, leading to the belief that it is the earliest mass produced acid, dating back to 3BC

Oxalic Acid is an organic acid with the chemical formula C₂H₂O₄. It is considered the simplest dicarboxylic acid, with it’s name being derived from the genus of the plant from which it was isolated, Oxalis. It occurs naturally in many foods, but too much can lead to kidney stones It is primarily used as a bleaching agent in cleaning solutions, rust removal, and teeth whitening. Oxalic acid has an oral lowest published lethal dose of 600 mg/kg. You can find detailed UV spectra of Oxalic Acid and information about its various lambda maxima by visiting the following link.

Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid can be separate and analyzed on a reverse-phase Newcrom BH column with a mobile phase consisting of water and perchloric acid as a buffer modifier. This analysis method can be UV detected at 200 nm.

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