HPLC Separation of cis- and trans – 1,4 cyclohexanedicarboxylic Acid on Newcrom BH Column

HPLC Method for 1,4-Cyclohexanedicarboxylic acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 1,4-Cyclohexanedicarboxylic acid.

1,4-Cyclohexanedicarboxylic acid is a pair of organic compounds with the chemical formula C8H12O4. It is typically used as a building block for polyesters and polyamides. Due to it improving hardness, flexibility, and impact strength of the previously mentioned compounds, it is used in automotive and construction industries. You can find detailed UV spectra of 1,4-Cyclohexanedicarboxylic acid and information about its various lambda maxima by visiting the following link.

1,4-Cyclohexanedicarboxylic acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.

Condition
Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 10/90%
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 200nm

 

Description
Class of Compounds Acid
Analyzing Compounds 1,4-Cyclohexanedicarboxylic acid

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
1,4-Cyclohexanedicarboxylic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.