HPLC Method for Acrylamide, Melamine on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acrylamide, Melamine.
Melamine is a nitrogen-based organic compound that was first synthesized by the German chemist Justus von Liebig in 1834. Melamine is a decorative coating that is resistant to water and mechanical damage.
Melamine is used for:
- lamination of particle boards and plywood;
- manufacture of paints and varnishes;
- production of decorative paper-laminated plastics and plastics;
- manufacture of dishes.
We were able to separate Melamine from Acrylamide in HPLC on Primesep 200 column using a mobile phase of water with Formic Acid buffer. This separation can be monitored with UV, ELSD, CAD detection techniques.
| Column | Primesep 200, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | H2O – 99% |
| Buffer | Formic Acid – 1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 240 nm |
| Class of Compounds | Amide, Amine |
| Analyzing Compounds | Acrylamide, Melamine |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
AcrylamideMelamine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
