HPLC MS Method for Analysis of Procarbazine Hydrochloride on Primesep 100 Column

HPLC Method for Procarbazine, Procarbazine hydrochloride on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Procarbazine, Procarbazine hydrochloride on Primesep 100 Column by SIELC Technologies

Procarbazine is a chemotherapy medication with the formula C12H19N3O. It is used primarily in treatment of Hodgkin lymphoma and brain cancers. Itison the World Health Organization’s List of Essential Medicines. In treatment of Hodgkin’s lymphoma, it is typically delivered as a part of the BEACOPP regiment, being the last “P” in the acronym. It is used in other combinations, usually as a part of PCV, when treating brain cancer. You can find detailed UV spectra of Procarbazine, Procarbazine hydrochloride and information about its various lambda maxima by visiting the following link.

Procarbazine, Procarbazine hydrochloride can be retained, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is carried out using UV

ColumnPrimesep 100, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 70/30%
BufferH2SO4  – 0.1%
Flow Rate0.2 ml/min
DetectionLC MS ESI SIM 222 (M+H)+
Class of CompoundsMedication
Analyzing CompoundsProcarbazine, Procarbazine hydrochloride

Application Column

Primesep 100

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Procarbazine
Procarbazine hydrochloride
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.