HPLC MS Method for Analysis of Amoxicillin on Primesep 100 Column

HPLC MS Method for Analysis of Procainamide on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Amoxicillin

Amoxicillin is a widely used antibiotic belonging to the penicillin class. It is used to treat a variety of bacterial infections, including respiratory tract infections, ear infections, skin infections, urinary tract infections, and certain types of bacterial endocarditis.

• Mechanism of Action: Amoxicillin works by inhibiting the synthesis of bacterial cell walls. It does this by interfering with the formation of peptidoglycan, an essential component of the bacterial cell wall.
• Spectrum of Activity: Amoxicillin is effective against a broad spectrum of bacteria, including both Gram-positive and Gram-negative bacteria. However, its effectiveness may be limited against bacteria that produce beta-lactamase enzymes.
• Administration: It is commonly available in oral formulations, such as capsules, tablets, and oral suspensions. There are also intravenous formulations for more severe infections.
• Common Uses:

• Respiratory Tract Infections: Amoxicillin is often prescribed for conditions like bronchitis and pneumonia.
• Ear Infections: It is used to treat middle ear infections.
• Skin Infections: Amoxicillin can be effective against various skin infections.
• Urinary Tract Infections (UTIs): It is sometimes prescribed for uncomplicated UTIs.

Amoxicillin can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 272 nm