HPLC MS Method for Analysis of Procainamide on Primesep 100 Column by SIELC Technologies

Amoxicillin is a prescription antibiotic with the molecular formula C₁₆H₁₉N₃O₅S. It is typically used to treat bacterial infections and is taken orally. It belongs to the penicillin class. It is used to treat a variety of bacterial infections, including respiratory tract infections, ear infections, skin infections, urinary tract infections, and certain types of bacterial endocarditis. Amoxicillin works by inhibiting the synthesis of bacterial cell walls. It does this by interfering with the formation of peptidoglycan, an essential component of the bacterial cell wall. It is effective against a broad spectrum of bacteria, including both Gram-positive and Gram-negative bacteria. However, its effectiveness may be limited against bacteria that produce beta-lactamase enzymes.

Amoxicillin can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 272 nm