HPLC Method for Analysis of Phenylephrine hydrochloride on Primesep S Column
Phenylephrine HCl is is a common over-the-counter decongestant, but can also be used for pupil dilation and hemorrhoid treatment. It’s chemical formula is C9H13NO2. It is sold under many brand names including Mucinex, Sudafed PE, Sinex, and many other generic brands. While it is best known as a decongestant, it has a wide variety of other pharmaceutical applications, including pupil dilation, hypotension (low blood pressure) treatment, and hemorrhoid relief. You can find detailed UV spectra of Phenylephrine hydrochloride and information about its various lambda maxima by visiting the following link.
Phenylephrine hydrochloride can be detected in the low UV regime. Using a Primesep S can be retained and analyzed. This analysis method can be UV detected at 220 nm.
| Column | Primesep S, 4.6 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 35% |
| Buffer | NaH2PO4 pH 4.5 – 2.6 g/l |
| Flow Rate | 2.0 ml/min |
| Detection | UV 220 nm |
| Retention Time | 4.12 min |
| Class of Compounds | Drug |
| Analyzing Compounds | Phenylephrine hydrochloride |
Application Column
Primesep S
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
