HPLC Method for Analysis of Pefloxacin on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pefloxacin on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Pefloxacin on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Pefloxacin

Pefloxacin is an antibiotic that belongs to the fluoroquinolone class of drugs. It is primarily used to treat bacterial infections by inhibiting the DNA gyrase enzyme, which is essential for bacterial DNA replication and repair. Because of its mechanism, Pefloxacin is effective against a variety of gram-negative and some gram-positive bacteria.

Pefloxacin can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.

You can find detailed UV spectra of Pefloxacin and information about its various lambda maxima by visiting the following link.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 50%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Limit of Detection40 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
quinolinemonocarboxylic acid
Analyzing CompoundsPefloxacin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Pefloxacin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.