HPLC Method for Analysis of Cytochrome C on BIST B+ by SIELC Technologies.
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analyses of Cytochrome C
Cytochrome C is a mitochondrial protein that is essential to both the respiratory electron transport chain and the apoptosis cycle of cells. Using SIELC’s newly introduced BIST™ method, this essential protein can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged amine analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Cytochrome C can be retained and UV detected at 395 nm.
Condition
| Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 395 nm |
| Peak Retention Time | 4.9 min |
Description
| Class of Compounds | Protein, Hemeprotein |
| Analyzing Compounds | Cytochrome C |
Application Column
BIST B
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
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BIST B+
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
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