Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Cytochrome C check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was diluted in water.

HPLC Method for Analysis of Cytochrome C on BIST B+ by SIELC Technologies.

Cytochrome C, also known as cytochrome complex, is a mitochondrial protein that is essential to both the respiratory electron transport chain and the apoptosis cycle of cells with the chemical formula C₄₂H₅₂FeN₈O₆S₂. It is a small hemeprotein that is usually associated with the inner membrane of the mitochondrion. It is primarily used to detect peroxide production in biological systems.

Using SIELC’s newly introduced BIST™ method, this essential protein can be retained on a positively-charged anion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged amine analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Cytochrome C can be retained and UV detected at 395 nm.

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