HPLC Method for Determination of Borate Ion in Boric Acid and Borax on Chromni Column

HPLC Method for Analysis of Orthoborate on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Orthoborate.

Sodium, which  has a chemical symbol of Na, is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

Borate ion, also known as Orthoborate, is a polyatomic anion with the formula BO33-. It is used primarily in the making of boric acid and orthoborate salts.

Orthoborate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using ELSD.

Condition

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O – 90/10
BufferAmFM pH 3.0 – 10 mM
Flow Rate1.0 ml/min
DetectionELSD, the nebulizer and evaporator temperatures
50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM)

Description

Class of CompoundsIons
Analyzing CompoundsOrthoborate

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Orthoborate
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.