HPLC Method for Xylylenediamine, m-Xylylenediamine on BIST B+ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Xylylenediamine, m-Xylylenediamine.
m-Xylylenediamine (MXDA) is a popular curing agent used on epoxy resins with the chemical formula C8H12N2. When MXDA undergoes the Sommelet reaction, it results in a isophthalaldehyde. You can find detailed UV spectra of m-Xylylenediamine and information about its various lambda maxima by visiting the following link.
Using SIELC’s newly introduced BIST method, Xylylenediamine, m-Xylylenediamine, which protonates in water, can be retained on a positively-charged anion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged MXDA analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, MXDA can be retained and UV detected at 210 nm.
| Column | BIST B+, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | H2SO4 – 0.2 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds |
Drug, Basic, Hydrophobic, Ionizable, Zwitterionic |
| Analyzing Compounds | Xylylenediamine, m-Xylylenediamine |
Application Column
BIST B+
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
m-Xylylenediamine
