HPLC Method for Tyramine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tyramine
Tyramine is an amine compound with the molecular formula C8H11NO. As a monoamine oxidase (MAO) substrate, it is broken down by the enzyme MAO in the body. It is typically found in fermented, aged, smoked, and dried foods.
Tyramine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Column | Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN – 50% |
Buffer | Sulfuric Acid |
Flow Rate | 0.5 ml/min |
Detection | UV 275 nm |
Class of Compounds | Amine |
Analyzing Compounds | Tyramine |
Application Column
Primesep 100
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended