HPLC Method for Analysis of Thymol Blue on Primesep 100 Column

HPLC Method for Thymol blue on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Thymol Blue

High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol blue 

Thymol Blue is a sulfonephthalein dye with C27H30O5S molecular formula. It is typically used as a pH indicator. Despite it’s name, it is only blue at higher pH levels, turning from blue to yellow when going basic to neutral and from yellow to red when changing from relatively acidic to very acidic. Due to the split in transition stages, it us considered a diprotic acid.

Thymol blue can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 25/75%
BufferFormic Acid – 0.3 %
Flow Rate1.0 ml/min
DetectionUV, 270 nm
Class of Compounds
Dye, Acid, Hydrophobic
Analyzing CompoundsThymol blue

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Thymol blue
Application Detection:
UV Detection
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