HPLC Method for Analysis of Sucralose on Primesep S2 Column

HPLC Method for Sucralose on Primesep S2 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Sucralose

Sucralose is an synthetic organochlorine compound with the molecular formula C12H19Cl3O8.

Properties:
Appearance: Typically a white, odorless, crystalline powder.

Molecular weight: ~397.6 g/mol

Solubility: Soluble in water.

Uses: An artificial sweetener used to provide sweetness in food and beverages without the calories of sugar. 

Sucralose can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Detection is performed using ELSD.

ColumnPrimesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 95%
BufferFormic Acid – 0.1%
Flow Rate1.0 ml/min
DetectionELSD
LOD3.152 ppm

Class of CompoundsChlorinated Disaccharide
Analyzing CompoundsSucralose

Application Column

Primesep S2

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Sucralose
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.