HPLC Method for Analysis of Propranolol on Primesep 100 Column on Alltesta™

HPLC Method for Propranolol on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Propranolol

Propranolol is an organic compound with the molecular formula C16H21NO2. It is primarily used to treat hypertension, angina, migraine headaches, and hypertrophic subaortic stenosis. As a beta-blocker, it works through affecting nerve impulse responses. You can find detailed UV spectra of Propranolol and information about its various lambda maxima by visiting the following link.

Propranolol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 70%
BufferSulfuric Acid
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsPhenols
Analyzing CompoundsPropranolol

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Propranolol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.