HPLC Method for Analysis of 10A Oligonucleotides on OligoMg Column by SIELC Technologies

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analyzing Oligo 10-mer Oligonucleotides on OligoMg Column by SIELC Technologies

HPLC Method for Analyzing 10-mer Oligonucleotides on OligoMg Column by SIELC Technologies

HPLC Method for Analyzing Oligo 10-mer Oligonucleotides

Oligo 10-mer oligonucleotides are short sequences of nucleotides consisting of ten nucleotide units. These oligonucleotides can be composed of any combination of the four standard nucleotides: adenine (A), thymine (T), cytosine (C), and guanine (G).

Research and Studies:

  • Molecular Biology: In molecular biology, such oligos can be used as primers for PCR, sequencing, or as probes in hybridization experiments.
  • Binding Studies: The poly(A) sequence may be used to study binding interactions with proteins, such as DNA-binding proteins or enzymes.

Medical and Diagnostic Applications:

  • As part of diagnostic kits, especially in assays that require hybridization to a complementary sequence.

Educational Purposes:

Demonstrating basic principles of nucleic acid chemistry and genetics.

Nanotechnology:

In DNA nanotechnology, specific sequences of DNA are used to form structures and shapes at the nanoscale. A poly(A) sequence might be part of a larger structure.

Using SIELC’s newly introduced BIST™ method, this oligonucleotide can be retained on a OligoMg column. Using this new and unique analysis method, oligonucleotide can be separated, retained, and detected at 260 nm.

Please read more on oligonucleotides analysis by HPLC in our June 2024 newsletter.

Condition

ColumnOligoMg, 4.6 x 100 mm, 3 µm, 100 A
Mobile PhaseGradient MeCN 70-45%, 10 min
Buffernone
Flow Rate1.0 ml/min
DetectionUV 260 nm

Description

Class of CompoundsOligonucleotides
Analyzing CompoundsOligonucleotides

Application Column

OligoMg

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A

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Application Analytes:
Oligonucleotides
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.