HPLC Method For Analysis Of Minocycline Hydrochloride on Newcrom R1 Column

HPLC Method for Minocycline hydrochloride on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Minocycline hydrochloride 

Minocycline hydrochloride is a tetracycline antibiotic with C23H27N3O7 • HCl [XH2O] molecular formula. It is used to treat a wide variety of bacterial infections, acne, and sexually transmitted infections.

Minocycline hydrochloride can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a triethanolamine buffer. Detection is performed using UV.

ColumnNewcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 5/95%
BufferTEAPh – 20 mM, pH 2.0
Flow Rate0.5 ml/min
DetectionUV, 270 nm
Class of Compounds
Drug, Antibiotics
Analyzing CompoundsMinocycline hydrochloride

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Minocycline hydrochloride
Application Detection:
UV Detection
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