HPLC Method for Analysis of m-Xylylenediamine (MXDA) on BIST™ B+ Column

HPLC Method for m-Xylylenediamine on BIST B+ by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of m-Xylylenediamine.

m-Xylylenediamine (MXDA) is a popular curing agent used on epoxy resins with the chemical formula C8H12N2. When MXDA undergoes the Sommelet reaction, it results in a isophthalaldehyde. You can find detailed UV spectra of m-Xylylenediamine and information about its various lambda maxima by visiting the following link.

Using SIELC’s newly introduced BIST™ method, m-Xylylenediamine, which protonates in water, can be retained on a positively-charged anion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged MXDA analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, MXDA can be retained and UV detected at 210 nm.

Condition
Column BIST B+, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 80%
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 210nm

Description
Class of Compounds Amine, Aliphatic amine
Analyzing Compounds m-Xylylenediamine

 

Application Column

BIST B+

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
m-Xylylenediamine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.