Glycine and glycine methyl ester hydrochloride are important amino acid compounds widely used in pharmaceutical, biochemical, and peptide research. Both compounds are water-soluble and play critical roles in protein synthesis, peptide modification, and metabolic studies. High-performance liquid chromatography (HPLC) offers a precise and reliable method to separate, identify, and quantify glycine and its methyl ester derivative with excellent sensitivity and reproducibility. This analytical approach is essential for quality control, pharmaceutical formulation analysis, and research applications involving amino acid derivatives. Using HPLC for these compounds ensures accurate, consistent results in laboratory and industrial settings.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Amino acid, Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Zwitterionic |
| Analyzing Compounds | Glycine, Glycine Methyl Ester |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsGlycine methyl ester
