HPLC Method for Glycine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glycine.
Glycine, also written as Gly or G, is an important amino acid compounds widely used in pharmaceutical, biochemical, and peptide research. It has the chemical formula C₂H₅NO₂. It is water-soluble and plays a critical role in protein synthesis, peptide modification, and metabolic studies. It can be found in meat., eggs. and bones. You can find detailed UV spectra of Glycine and information about its various lambda maxima by visiting the following link.
Glycine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with sulfuric acid as a buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 10% |
| Buffer | Sulfuric Acid – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |
| LOD* | 6 ppm |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Amino Acids |
| Analyzing Compounds | Glycine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
