HPLC Method for Diuron on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Diuron
Diuron, also known as DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea), is an algicide and herbicide with the chemical formula C9H10Cl2N2O. It works through inhibiting photosynthesis primarily broadleaf weeds such as crabgrass, foxtail, and pigweed. It is toxic when ingested and there are studies being conducted to test for whether or not it is a carcinogen. You can find detailed UV spectra of Diuron and information about its various lambda maxima by visiting the following link.
Diuron can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 50% |
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Herbicide |
| Analyzing Compounds | Diuron |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
