HPLC Method For Analysis Of Dimethylformamide and Dimethylacetamide on Primesep 100 Column

HPLC Method for DMF (Dimethylformamide), DMA (Dimethylacetamide) on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of DMF (Dimethylformamide), DMA (Dimethylacetamide).

Dimethylformamide, also written as DMF, is an organic compound with the chemical formula HCON(CH3)2. It is primarily used as a solvent for chemical reactions due to it’s low evaporation rate. It is used in the production of acrylic fibers, adhesives, synthetic leathers, and plastics.  Pharmaceutically, it is used as a solvent in peptide coupling and the production of pesticides.

Dimethylacetamide, also written as DMAc and DMA, is an organic compound with the chemical formula C4H9NO. It is used primarily as a polar solvent in organic synthesis. Since 2014, DMA has been under investigation due to it’s toxicity.

DMF (Dimethylformamide), DMA (Dimethylacetamide) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 5/95%
Buffer H3PO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV, 200 nm,

Description
Class of Compounds
 Amide
Analyzing Compounds DMF (Dimethylformamide), DMA (Dimethylacetamide)

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
DMA (Dimethylacetamide)
DMF (Dimethylformamide)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.