HPLC Method for Analysis of Caffeic Acid on Primesep 100 Column on Alltesta™

HPLC Method for Caffeic acid on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Caffeic acid 

Caffeic acid is an organic compound with the formula  (HO)2C6H3CH=CHCO2H. It plays a key role in scavenging reactive oxygen species (ROS) that are generated during energy metabolism.  Caffeic acid is an active ingredient in caffenol, which is a photographic developer made from instant coffee. Pharmacologically, it is used  as an antioxidant in vitro and also in vivo. It also shows immunomodulatory and anti-inflammatory activity. You can find detailed UV spectra of Caffeic acid and information about its various lambda maxima by visiting the following link.

Caffeic acid can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN -20/80%
BufferH3PO4 – 0.5%
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsAcid
Analyzing CompoundsCaffeic acid

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Caffeic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.