B-Estradiol is an estrogen steroid hormone. It is the major estrogen secreted by the premenopausal ovary. It is synthesized from testosterone primarily in the ovarian granulosa cells and placenta. B-estradiol is used as a medication, for instance in menopausal hormone therapy. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain B-Estradiol. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Primesep 200, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 5-50%, 5 min, 4 min hold |
| Buffer | Gradient H2SO4 – 0.05-0.3 %, 5 min, 4 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Hormone, Hydrophobic |
| Analyzing Compounds | B-Estradiol |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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