HPLC Method for Analysis of Ataciguat on Primesep 100 Column

HPLC Method for Analysis of Ataciguat on Primesep 100 Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ataciguat.

Ataciguat is an drug with the chemical formula C21H19N3O6S3. It is primarily used as a guanylate cyclase activator. Through activating sGC and restoring the NO-cGMP pathways, it helps to reduce calcium deposition, slow valve calcification, and generally treats calcific aortic valve stenosis. t helps reduce calcium deposition You can find detailed UV spectra of Ataciguat and information about its various lambda maxima by visiting the following link.

Ataciguat can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using UV.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 50/50 – 80/20%, 10 min
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 241 nm
Limit Of Detection*150 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsDrug
Analyzing CompoundsAtaciguat

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Ataciguat
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.