HPLC Method for Analysis of Ascorbic Acid on Primesep SB Column on Cromite™

HPLC Method for Ascorbic Acid on Primesep SB by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Ascorbic Acid

Ascorbic Acid is a vitamin with the molecular formula C6H8O6. Typically, it is used to treat scurvy, support immune system, and preserve food. It is a white to light yellow powder that is easily dissolved in water. It can be found in a large variety of fruits and vegetables, especially in citrus fruits.

Ascorbic Acid can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferSulfuricAcid
Flow Rate0.5 ml/min
DetectionUV 275 nm

Class of CompoundsVitamin
Analyzing CompoundsAscorbic Acid

Application Column

Primesep SB

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Ascorbic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.