HPLC ELSD Method for Analysis of Aminomethylphosphonic acid (AMPA) on Chromni Column

HPLC Method for Analysis of Aminomethylphosphonic acid (AMPA) on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Aminomethylphosphonic acid (AMPA).

Aminomethylphosphonic Acid is an aminophosphonate with the chemical formula CH6NO3P. It is primarily used as a biocide and pesticide, as well as in research to assess the exposure of glyphosate. It is a major toxic metabolite of glyphosate as is considered to be a similar toxicological concern. You can find detailed UV spectra of Aminomethylphosphonic acid (AMPA) and information about its various lambda maxima by visiting the following link.

Aminomethylphosphonic acid (AMPA) can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocraticmethod with a simple mobile phase consisting of water and acetonitrile (MeCN) and TFA. Detection is performed using ELSD.

Condition

ColumnChromni, 2.1 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O – 80/20%
BufferTFA – 0.2%
Flow Rate0.2 ml/min
DetectionELSD
Limit Of Detection*300 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsAcid
Analyzing CompoundsAminomethylphosphonic acid (AMPA)

Application Column

Chromni

Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

Add to cart
Application Analytes:
Aminomethylphosphonic acid (AMPA)
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.