HPLC Method for Alprazolam on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Alprazolam
Alprazolam, also known as Xanax, is a perscription medication with the C17H13ClN4 molecular formula. It is typically used to treat anxiety and panic disorders. It works through enhancing the effects of GABA, which is a calming neurotransmitter in the brain.
Alprazolam can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN/H2O – 30/70% |
Buffer | H3PO4 – 0.2% |
Flow Rate | 0.5 ml/min |
Detection | UV, 250 nm |
Class of Compounds | Drug, Sedative, Hydrophobic, Neutral, Acid |
Analyzing Compounds | Alprazolam |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended