HPLC Method for Simazine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Simazine
Simazine is an herbicide with the molecular formula C7H12ClN5. Typically, it is used to control various annual weeds in established crops. It is a pre-emergence herbicide, meaning that it has to be applied prior to the weeds emerging, as it is ineffective against grown plants
Simazine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN – 50% |
Buffer | H2SO4 – 0.2% |
Flow Rate | 1.0 ml/min |
Detection | UV 275 nm |
Class of Compounds | Herbicide |
Analyzing Compounds | Simazine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended