HPLC Method for Analysis of 5-Aminoindol on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of 5-Aminoindole on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of 5-Aminoindol on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of 5-Aminoindole

5-Aminoindole is an organic compound with the chemical formula C8H8N2. It is a derivative of indole and features an amino group at the 5-position.

Applications:
Pharmaceuticals: It can serve as a building block in the synthesis of various pharmaceutical compounds.
Research: Used in studies related to indole derivatives, which are important in medicinal chemistry.
5-Aminoindole be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 55%
BufferH2SO4 -0.05%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Samples1.0 mg/ml MeCN/H2O – 50/50%
Injection volume1 µl
LOD*30 ppb (200 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Pyridines
Analyzing Compounds5-Aminoindole

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
5-Aminoindole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.