HPLC-MS Method for Analysis of Cariprazine on Primesep B Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cariprazine on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Cariprazine on Primesep B Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Cariprazine

Cariprazine is an atypical antipsychotic medication primarily used to treat psychiatric disorders such as schizophrenia and bipolar disorder. It is a dopamine D2/D3 receptor partial agonist, with a preference for the D3 receptor, which helps modulate neurotransmitter activity in the brain. This unique mechanism makes it effective in managing symptoms like hallucinations, delusions, and mood swings.

Brand name: Vraylar (most common)

  • Drug class: Atypical antipsychotic
  • Mechanism of action: Partial agonist at dopamine D2 and D3 receptors, with some serotonin receptor (5-HT1A) agonism and 5-HT2B/5-HT2A antagonism.

Advantages:

Cariprazine has shown efficacy in treating both the positive (hallucinations, delusions) and negative (social withdrawal, lack of motivation) symptoms of schizophrenia. Its partial agonist effect at D3 receptors may help reduce the risk of some side effects seen with other antipsychotics, such as weight gain or sedation.

Cariprazine can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and formic acid as a buffer. This method allows for detection using UV 260 nm.

You can find detailed UV spectra of Cariprazine and information about its various lambda maxima by visiting the following link.

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN/H2O – 30-70%, 10 min
BufferFormic Acid – 0.2%
Flow Rate1.0 ml/min
DetectionUV 260 nm, LC-MS (ESI, SIM) m/z 247 [M+]
Limit of DetectionUV 40 ppb, LC MS – 3 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Drug
Analyzing CompoundsCariprazine

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Cariprazine
Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.