HPLC Method for Analysis of Biuret on Primesep S Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Biuret on Primesep S by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Biuret on Primesep S Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Biuret

Biuret, with the chemical formula C₂H₅N₃O₂, is a white crystalline compound formed by the condensation of two urea molecules with the release of ammonia. Its structure consists of two amide groups (-CONH) linked by a nitrogen atom. Biuret is widely used in biochemistry for protein assays, specifically in the biuret test, which detects peptide bonds. Due to its polar functional groups, the compound is highly soluble in water.

Biuret can be retained, separated and analyzed using a Primesep S mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric as a buffer. This method allows for detection using UV 200 nm.

ColumnPrimesep S, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 90%
BufferH3PO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 200 nm

Class of Compounds
Condensed Ureas
Analyzing CompoundsBiuret

Application Column

Primesep S

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Biuret
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.