HPLC ELSD Method for Simultaneous Determination of Magnesium (II) and Sulfate ions on Chromni Column

HPLC Method for Analysis of Magnesium, Sulfate on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Magnesium, Sulfate.

Magnesium is an element with the symbol Mg. Magnesium ion has the chemical formula of Mg2+. It is a shiny grey metal with a high chemical reactivity, but low density and melting point. While magnesium is abundant in the earth’s crust, it is produced in the cosmos in large, aging stars. It is primarily used as a structural metal in a large variety of contexts from aircrafts to light sources and chemical reagents.

Sulfate is an ionic chemical with the formula SO42-. In nature, it is usually found in geodes like gypsum. It is also created in atmosphere from sulfur dioxide. It is used for a variety of applications, but most notably, coloring wood and creating light-reflecting paints.

Magnesium, Sulfate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using LC MS.

Condition

ColumnChromni, 3.2 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O
BufferTFA – 0.2%
Flow Rate0.5 ml/min
DetectionELSD, the nebulizer and evaporator temperatures
50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
LOD*Mg2+ – 10 ppm, SO₄²⁻ – 20 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsIons
Analyzing CompoundsMagnesium, Sulfate

Application Column

Chromni

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Magnesium
Sulfate
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.