HPLC Method for Nitrate, Phosphate, Selenate on Chromni by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Nitrate, Phosphate, Selenate.
Nitrate, NO3–, is a compound of nitrogen and oxygen. It is essential as a nutrient in plants; therefore, it is often used in fertilizers. It is easily found in leafy greens. While it is important for cardiovascular health, too high of exposure to it can be dangerous.
Phosphate, PO43-, is a compound that plays an important part in biological energy transfer. It contains phosphorus, which is also a key component in bones and teeth. It is also a buffer, which helps maintain pH levels in a body.
Selenate is an ion of selenium with the chemical formula SeO32-. It is a good oxidizer, easily reduced to selenite and selenium.
Nitrate, Phosphate, Selenate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using ELSD.
| Column | Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated |
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | AmFm pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
Application Column
Chromni
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated
Phosphate
Selenate
