High Performance Liquid Chromatography (HPLC) Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
Pararosaniline (Basic Red 9) is a popular basic magenta dye and part of the triarylmethane family of dyes. Crystal Violet (Methyl Violet 10B), another basic triarylmethane dye, is frequently used for histological stains and for identifying Gram-positive bacteria. Crystal Violet Lactone, a derivative of Crystal Violet, is a basic thermochromic dye widely used as a security marker for various types of fuels. These three basic dyes can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV-Vis regime at 540, 590, and 200 nm.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Pararosaniline, Crystal Violet and Crystal Violet Lactone
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 540, 590, 200 nm |
| Peak Retention Time | 3.15, 7.24, 8,82 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Pararosaniline, Crystal Violet and Crystal Violet Lactone |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsCrystal Violet Lactone
Pararosaniline Hydrochloride
