HPLC Determination of Oxalic Acid on Newcrom B Column

HPLC Method for Oxalic Acid on Newcrom B by SIELC Technologies

HPLC Determination of Oxalic on Newcrom B Column_1196

High Performance Liquid Chromatography (HPLC) Method for Analysis of Oxalic Acid 

Oxalic Acid. also known as ethanedioic acid is an organic acid. It occurs naturally in plants, but is also often found in industrial uses as a bleaching agent, rust remover, and cleaning agent. Industrial uses of it also extend into households, as it is easily accessible. Despite that, in large amounts, it can be toxic.

Oxalic Acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.

Condition

Column Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 20/80%
Buffer Formic Acid – 2.0 %
Flow Rate 1.0 ml/min
Detection CAD (Corona)

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Oxalic Acid

 

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Oxalic Acid

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.