HPLC Method for Analysis of Mellitic acid on Newcrom BH Column by SIELC Technologies
Separation type: Liquid Chromatography Reversed-phase
Mellitic Acid is an aromatic dicarboxylic acid with the chemical formula C12H6O12. It is a white crystalline solid and is derived from benzoic acid. Mellitic acid is named after mellite, a mineral in which it was first discovered. It is not commonly encountered in everyday use and does not have widespread applications.
Chemical Structure: Mellitic acid consists of a benzene ring with six carboxylic acid functional groups (-COOH) attached to it, forming a hexacarboxylic acid.
Occurrence: Mellitic acid is not a naturally abundant compound and is primarily known for its existence as a mineral, mellite, which contains mellitic acid.
Uses: Due to its structure and properties, mellitic acid does not have significant practical applications in industry or daily life. It is more commonly encountered in scientific contexts and research.
Derivatives: Mellitic acid can form various derivatives, and some of its salts and esters have been studied for specific purposes.
Mellitic acid is mainly of interest in the fields of chemistry and mineralogy, and its practical applications are limited compared to other acids.
Mellitic acid can be retained and analyzed on a reversed-phase Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 220 nm using UV detection
High Performance Liquid Chromatography (HPLC) Method for Analyses of Mellitic acid on Newcrom BH Column by SIELC Technologies
Condition
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 220 nm |
| Injection volume: | 1 µL |
| Sample | 0.25 mg/ml |
| Sample Diluent | H2O – 100% |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Mellitic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
