HPLC Method for Analysis of Mellitic Acid on Newcrom BH Column

HPLC Method for Analysis of Mellitic acid on Newcrom BH Column by SIELC Technologies

HPLC Method for Analysis of Mellitic acid on Newcrom BH Column by SIELC Technologies

Mellitic Acid is an aromatic dicarboxylic acid with the chemical formula C12H6O12. It is a white crystalline solid and is derived from benzoic acid. Mellitic acid is named after mellite, a mineral in which it was first discovered. It is not commonly encountered in everyday use and does not have widespread applications. It is more commonly encountered in scientific contexts and research. Mellitic acid can form various derivatives, and some of its salts and esters have been studied for specific purposes. It consists of a benzene ring with six carboxylic acid functional groups (-COOH) attached to it, forming a hexacarboxylic acid. Mellitic acid is not a naturally abundant compound and is primarily known for its existence as a mineral, mellite, which contains mellitic acid.

Mellitic acid can be retained and analyzed on a reversed-phase Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 220 nm using UV detection

Condition

ColumnNewcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 220 nm
Injection volume:1 µL
Sample0.25 mg/ml
Sample DiluentH2O – 100%

Description

Class of CompoundsAcid
Analyzing CompoundsMellitic acid

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Mellitic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.