HPLC Determination of DO3A on Primesep 200 Column

 

HPLC Method for DO3A on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of DO3A.

DO3A is a derrivative of DOTA and has the chemical formula C₁₄H₂₃N₄O₆Na₃. DOTA is an acronym for 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid. It is used to bind metal ions, partially due to it’s stability. It is often employed in biomedical applications including but not limited to MRI contrast agents and targeted cancer therapy.

DO3A can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.

Condition
Column Primesep 200
Mobile Phase MeCN/H2O – 30/70%
Buffer H2SO4 – 0.02 %
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description
Class of Compounds
 Acid, Hydrophilic, Ionizable, Carboxylic acid, Drug, Carbocyclic.
Analyzing Compounds DO3A

 

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
DO3A
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.