Coenzyme Q10, aka ubiquinone, is an enzyme found in animals and is used as a dietary supplement. Ubiquinol is a reduced form of coenzyme Q10. The two compounds are similar in structure and can be difficult to separate in reverse-phase HPLC. Using mixed-mode Primesep D column and the mobile phase with acetonitrile (ACN) and water, without the need for buffer, CoQ10 and ubiquinol can be isocratically separated and UV detected at 275nm.
| Column | Primesep D |
| Mobile Phase | MeCN/H2O – 95/5% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm, Vis 435 nm |
| Class of Compounds | Hydrophobic |
| Analyzing Compounds | Coenzyme Q10, Ubiquinol |
Application Column
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsUbiquinol
