HPLC Method for Amlodipine besylate on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Amlodipine besylate
Amlodipine is a prescription typically sold under the brand name “Norvasc.” It is used to treat high blood pressure and artery disease. It works by preventing calcium from entering cells through blocking L-type calcium channels.
Amlodipine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H2SO4 – 0.1 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 238 nm |
| Class of Compounds |
Drug, Hydrophobic, Ionizable, Zwitterionic |
| Analyzing Compounds | Amlodipine besylate |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Amlodipine besylateApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
