HPLC Determination of 6-Aminocaproic Acid With Low UV Detection

HPLC Method for 6-Aminocaproic acid on Newcrom AH by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Aminocaproic acid.

6-Aminocaproic Acid, brand name Amicar, is a clotting agent used to control excessive bleeding in patients during or after surgery by acting as an inhibitor to fibrinolysis. It is also used to treat  a variety of disorders from Thrombocytopenia to bleeding disorders. It’s chemical formula is C6H13NO2. You can find detailed UV spectra of 6-Aminocaproic Acid and information about its various lambda maxima by visiting the following link.

It can be retained using HPLC on Newcrom AH mixed-mode column using isocratic analysis with mobile phase consisting of acetonitrile and water (ACN/H2O) with sulfuric acid (H2SO4) as buffer and detected at low UV of 200 nm.

Condition
Column Newcrom АH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 200 nm

Description
Class of Compounds Acid
Analyzing Compounds 6-Aminocaproic acid

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
6-Aminocaproic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.