HPLC Determination of 1,5-Naphthalenedisulfonic Acid on Newcrom B Column

HPLC Method for 1,5-Naphthalenedisulfonic Acid on Newcrom B by SIELC Technologies

 

 

Condition 1
Column Newcrom B, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV 230nm

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of 1,5-Naphthalenedisulfonic Acid.

1,5-Naphthalenedisulfonic acid, also known as Armstrong’s acid, is a strong organic acid with a double charge. It has the chemical formula C10H8S2O6.

Multi-charged molecules generally have a tendency to exhibit poor peak shape in HPLC on reverse-phase columns showing significant tailing.  1,5-Naphthalenedisulfonic Acid can be retained with great peak shape on a Newcrom B mixed-mode column.  The Newcrom line of columns was designed specifically for improved peak shape of multi-charged molecules.  The method uses a mobile phase consisting of acetonitrile (ACN), water and ammonium formate buffer, making the method MS-compatible.  UV detection at 260nm can also be used.

Condition 2
Column Newcrom B, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 70/30%
Buffer Ammonium Formate pH 3.0 – 40 mM
Flow Rate 0.5 ml/min
Detection UV 285 nm

Description
Class of Compounds Acid
Analyzing Compounds 1,5-Naphthalenedisulfonic Acid

 

Application Column

Newcrom B

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
1,5-Naphthalenedisulfonic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.