HPLC Chiral Separation of Viloxazine Enantiomers on Cyralis CL Column

HPLC Chiral Method for Analysis of Viloxazine Enantiomers on Cyralis CL Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Viloxazine.

Viloxazine is a selective norepinephrine reuptake inhibitor with the chemical formula C13H19NO3. It is used in treatment of attention deficit hyperactivity disorder (ADHD). Originally, it was used to treat depression, but was discontinued. You can find detailed UV spectra of Viloxazine and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Viloxazine enantiomers using SIELC’s Cyralis CL chiral column. Cyralis CL is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Viloxazine can be retained and analyzed using the Cyralis CL stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of sodium perchlorate and acetonitrile. Detection is performed using UV.

Condition

ColumnCyralis CL, 4.6 x 100 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/Ethanolamine – 50/50/0.5%
Flow Rate0.5 mL/min
DetectionUV 230 nm
Resolution2.14

Description

Class of CompoundsDrug
Analyzing CompoundsViloxazine

Application Column

Cyralis CL

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Viloxazine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.