Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Fitusiran check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis Fitusiran on OligoMg Column by SIELC Technologies

Fitusiran is a relatively new therapeutic agent developed for the treatment of hemophilia A and B, including in patients with or without inhibitors.

Using SIELC’s newly introduced BIST™ method, this oligonucleotide can be retained on a OligoMg column. Using this new and unique analysis method, oligonucleotide can be separated, retained, and detected at 260 nm.

Please read more on oligonucleotides analysis by HPLC in our June 2024 newsletter.

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